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KMID : 0613820110210050621
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Journal of Life Science
2011 Volume.21 No. 5 p.621 ~ p.625
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AMPK γ is Required for Maintaining Epithelial Cell Structure and Polarity
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Koh Hyong-Jong
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Abstract
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AMP-activated protein kinase (AMPK), a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits, has been primarily studied as a major metabolic regulator in various organisms, but recent genetic studies discover its novel physiological functions. The first animal model with no functional AMPK γ subunit gene was generated by using Drosophila genetics. AMPK γ null flies demonstrated lethality with severe defects in cuticle formation. Further histological analysis found that deletion of AMPK γ causes severe defects in cell polarity in embryo epithelia. The phosphorylation of nonmuscle myosin regulatory light chain (MRLC), a critical regulator of epithelial cell polarity, was also diminished in AMPK γ null embryo epithelia. These defects in AMPK γ mutant epithelia were successfully restored by over-expression of AMPK γ. Collectively, these results suggested that AMPK γ is a critical cell polarity regulator in metazoan development.
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KEYWORD
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Drosophila, AMPK γ, MRLC, epithelia, cell polarity
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